merylはk-merカウントを行うツール。Celera Assemblerのために書かれた'meryl'をほぼ全面的に書き直したものが公開されている。
マニュアル
https://meryl.readthedocs.io/en/latest/index.html
インストール
ビルド依存
- gcc 7.4.0 or higher
##from release
wget https://github.com/marbl/meryl/releases/download/v1.3/meryl-1.3.Linux-amd64.tar.xz
tar -xJf meryl-1.3.Linux-amd64.tar.xz
export PATH=/path/to/meryl-1.3/build/bin:$PATH
#from source
git clone https://github.com/marbl/meryl.git
cd meryl/src
make -j 24
export PATH=/path/to/meryl/*/bin:$PATH
> meryl
usage: meryl ...
A meryl command line is formed as a series of commands and files, possibly
grouped using square brackets. Each command operates on the file(s) that
are listed after it.
COMMANDS:
statistics display total, unique, distnict, present number of the kmers on the screen. accepts exactly one input.
histogram display kmer frequency on the screen as 'frequency<tab>count'. accepts exactly one input.
print display kmers on the screen as 'kmer<tab>count'. accepts exactly one input.
count Count the occurrences of canonical kmers in the input. must have 'output' specified.
count-forward Count the occurrences of forward kmers in the input. must have 'output' specified.
count-reverse Count the occurrences of reverse kmers in the input. must have 'output' specified.
k=<K> create mers of size K bases (mandatory).
n=<N> expect N mers in the input (optional; for precise memory sizing).
memory=M use no more than (about) M GB memory.
threads=T use no more than T threads.
compress compress homopolymer runs to a single letter.
less-than N return kmers that occur fewer than N times in the input. accepts exactly one input.
greater-than N return kmers that occur more than N times in the input. accepts exactly one input.
equal-to N return kmers that occur exactly N times in the input. accepts exactly one input.
not-equal-to N return kmers that do not occur exactly N times in the input. accepts exactly one input.
increase X add X to the count of each kmer.
decrease X subtract X from the count of each kmer.
multiply X multiply the count of each kmer by X.
divide X divide the count of each kmer by X.
divide-round X divide the count of each kmer by X and round results. count < X will become 1.
modulo X set the count of each kmer to the remainder of the count divided by X.
union return kmers that occur in any input, set the count to the number of inputs with this kmer.
union-min return kmers that occur in any input, set the count to the minimum count
union-max return kmers that occur in any input, set the count to the maximum count
union-sum return kmers that occur in any input, set the count to the sum of the counts
intersect return kmers that occur in all inputs, set the count to the count in the first input.
intersect-min return kmers that occur in all inputs, set the count to the minimum count.
intersect-max return kmers that occur in all inputs, set the count to the maximum count.
intersect-sum return kmers that occur in all inputs, set the count to the sum of the counts.
subtract return kmers that occur in the first input, subtracting counts from the other inputs
difference return kmers that occur in the first input, but none of the other inputs
symmetric-difference return kmers that occur in exactly one input
MODIFIERS:
output O write kmers generated by the present command to an output meryl database O
mandatory for count operations.
EXAMPLES:
Example: Report 22-mers present in at least one of input1.fasta and input2.fasta.
Kmers from each input are saved in meryl databases 'input1' and 'input2',
but the kmers in the union are only reported to the screen.
meryl print \
union \
[count k=22 input1.fasta output input1] \
[count k=22 input2.fasta output input2]
Example: Find the highest count of each kmer present in both files, save the kmers to
database 'maxCount'.
meryl intersect-max input1 input2 output maxCount
Example: Find unique kmers common to both files. Brackets are necessary
on the first 'equal-to' command to prevent the second 'equal-to' from
being used as an input to the first 'equal-to'.
meryl intersect [equal-to 1 input1] equal-to 1 input2
実行方法
ゲノムのfastaファイルとk値、出力ディレクトリを指定する。
meryl count k=15 output merylDB genome.fa.gz
merylDB/に結果は保存される。
引用
GitHub - marbl/meryl: A genomic k-mer counter (and sequence utility) with nice features.
参考
https://hpc.nih.gov/apps/Meryl.html
